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Optimizing recombinant antibodies for intracellular function using hitchhiker-mediated survival selection
The ‘hitchhiker’ mechanism of the bacterial twin-arginine translocation pathway has previously been adapted as a genetic selection for detecting pairwise protein interactions in the cytoplasm of living Escherichia coli cells. Here, we extended this method, called FLI-TRAP, for rapid isolation of int...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4191445/ https://ncbi.nlm.nih.gov/pubmed/25225416 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzu038 |
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