Optimizing recombinant antibodies for intracellular function using hitchhiker-mediated survival selection

The ‘hitchhiker’ mechanism of the bacterial twin-arginine translocation pathway has previously been adapted as a genetic selection for detecting pairwise protein interactions in the cytoplasm of living Escherichia coli cells. Here, we extended this method, called FLI-TRAP, for rapid isolation of int...

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Bibliografiset tiedot
Päätekijät: Waraho-Zhmayev, Dujduan, Meksiriporn, Bunyarit, Portnoff, Alyse D., DeLisa, Matthew P.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Oxford University Press 2014
Aiheet:
Creativity Engenders Next-Generation Antibody Engineering
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4191445/
https://ncbi.nlm.nih.gov/pubmed/25225416
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzu038
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