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Versatile selection technology for intracellular protein–protein interactions mediated by a unique bacterial hitchhiker transport mechanism

We have developed a reliable genetic selection strategy for isolating interacting proteins based on the “hitchhiker” mechanism of the Escherichia coli twin-arginine translocation (Tat) pathway. This method, designated FLI-TRAP (functional ligand-binding identification by Tat-based recognition of ass...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
Main Authors: Waraho, Dujduan, DeLisa, Matthew P.
פורמט: Artigo
שפה:Inglês
יצא לאור: National Academy of Sciences 2009
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC2656142/
https://ncbi.nlm.nih.gov/pubmed/19234130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0704048106
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