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Trapping and Characterization of a Reaction Intermediate in Carbapenem Hydrolysis by B. cereus Metallo-β-lactamase

Metallo-β-lactamases hydrolyze most β-lactam antibiotics. The lack of a successful inhibitor for them is related to the previous failure to characterize a reaction intermediate with a clinically useful substrate. Stopped-flow experiments together with rapid freeze-quench EPR and Raman spectroscopies...

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Detalhes bibliográficos
Main Authors: Tioni, Mariana F., Llarrull, Leticia I., Poeylaut-Palena, Andrés A., Martí, Marcelo A., Saggu, Miguel, Periyannan, Gopal R., Mata, Ernesto G., Bennett, Brian, Murgida, Daniel H., Vila, Alejandro J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2645938/
https://ncbi.nlm.nih.gov/pubmed/18980308
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja801169j
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