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Direct Observation of Hierarchical Folding in Single Riboswitch Aptamers

Riboswitches regulate genes through structural changes in ligand-binding RNA aptamers. Using an optical-trapping assay based on in situ transcription by a molecule of RNA polymerase, single nascent RNAs containing pbuE adenine riboswitch aptamers were unfolded and refolded. Multiple folding states w...

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Hlavní autoři: Greenleaf, William J., Frieda, Kirsten L., Foster, Daniel A. N., Woodside, Michael T., Block, Steven M.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2640945/
https://ncbi.nlm.nih.gov/pubmed/18174398
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1151298
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