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Direct Observation of Hierarchical Folding in Single Riboswitch Aptamers
Riboswitches regulate genes through structural changes in ligand-binding RNA aptamers. Using an optical-trapping assay based on in situ transcription by a molecule of RNA polymerase, single nascent RNAs containing pbuE adenine riboswitch aptamers were unfolded and refolded. Multiple folding states w...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2640945/ https://ncbi.nlm.nih.gov/pubmed/18174398 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1151298 |
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