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Direct Observation of Cotranscriptional Folding in an Adenine Riboswitch

Growing RNA chains fold cotranscriptionally as they are synthesized by RNA polymerase (RNAP). Riboswitches, which regulate gene expression by adopting alternative RNA folds, are sensitive to cotranscriptional events. We developed an optical-trapping assay to follow the cotranscriptional folding of a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Frieda, Kirsten L., Block, Steven M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3496414/
https://ncbi.nlm.nih.gov/pubmed/23087247
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1225722
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