Expression, purification, crystallization and preliminary X-ray diffraction analysis of the transcriptional repressor SirR from Mycobacterium tuberculosis H37Rv

SirR, a metal-dependent transcriptional repressor from Mycobacterium tuber­culosis (Rv2788), was cloned in pQE30 expression vector with an N-­terminal His(6) tag for heterologous overexpression in Escherichia coli M15 (pREP4) cells and purified to homogeneity using chromatographic procedures. The pu...

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Bibliographic Details
Main Authors: Saha, Baisakhee, Mukherjee, Somnath, Dutta, Debajyoti, Das, Amit Kumar
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2009
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2635875/
https://ncbi.nlm.nih.gov/pubmed/19194009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108043534
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