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Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of Rv0241c (HtdX) from Mycobacterium tuberculosis H37Rv

Rv0241c (HtdX) is a putative (3R)-hydroxyacyl-CoA dehydratase of Mycobacterium tuberculosis. The htdX gene belongs to a conserved operon and is expressed in mycobacteria in the presence of several fatty-acid synthase II drugs. To elucidate the structure of HtdX, the protein was cloned, overexpressed...

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書目詳細資料
Main Authors: Biswas, Rupam, Dutta, Debajyoti, Das, Amit Kumar
格式: Artigo
語言:Inglês
出版: International Union of Crystallography 2013
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3792668/
https://ncbi.nlm.nih.gov/pubmed/24100560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113023452
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