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Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of an atypical two-cysteine peroxiredoxin (SAOUHSC_01822) from Staphylococcus aureus NCTC 8325

An atypical two-cysteine peroxidase, SAOUHSC_01822, from the virulent Staphylococcus aureus strain NCTC 8325 plays a major role in the reponse of the bacterium to oxidative stress. The protein was cloned, expressed, purified to homogeneity and crystallized. The protein was crystallized from 2 M ammo...

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Bibliografski detalji
Glavni autori: Bhattacharyya, Sudipta, Dutta, Debajyoti, Ghosh, Ananta Kumar, Das, Amit Kumar
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2009
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2777037/
https://ncbi.nlm.nih.gov/pubmed/19923729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109037580
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