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Expression, purification, crystallization and preliminary X-ray diffraction studies of phosphoglycerate mutase from Staphylococcus aureus NCTC8325

Phosphoglycerate mutase (PGM) is a key enzyme in carbohydrate metabolism. It takes part in both glycolysis and gluconeogenesis. PGM from pathogenic Staphylococcus aureus (NCTC8325) was cloned in pQE30 expression vector overexpressed in Escherichia coli M15 (pREP4) cells and purified to homogeneity....

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Bibliografiske detaljer
Main Authors: Roychowdhury, Amlan, Kundu, Anirban, Gujar, Akanksha, Bose, Madhuparna, Das, Amit Kumar
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3943096/
https://ncbi.nlm.nih.gov/pubmed/24419617
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X13031476
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