Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of glyceraldehyde-3-phosphate dehydrogenase from Antheraea mylitta

Glyceraldehyde-3-phosphate dehydrogenase from Antheraea mylitta (AmGAPDH) was cloned in pQE30 vector, overexpressed in Escherichia coli M15 (pREP4) cells and purified to homogeneity. The protein was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to the orthorhombi...

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Detalhes bibliográficos
Main Authors: Mukherjee, Somnath, Maity, Samita, Roy, Sobhan, Ghorai, Suvankar, Chakrabarti, Mrinmay, Agarwal, Rachit, Dutta, Debajyoti, Ghosh, Ananta Kumar, Das, Amit Kumar
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2795606/
https://ncbi.nlm.nih.gov/pubmed/19724138
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910903214X
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