Expression, purification, crystallization and preliminary X-ray diffraction studies of glyceraldehyde-3-phosphate dehydrogenase 1 from methicillin-resistant Staphylococcus aureus (MRSA252)

Glyceraldehyde-3-phosphate dehydrogenase 1 from methicillin-resistant Staphylo­coccus aureus (MRSA252) was cloned in pQE30 vector, overexpressed in Escherichia coli M15(pREP4) cells and purified to homogeneity. The protein was crystallized using the hanging-drop vapour-diffusion method. The crystals...

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Detalhes bibliográficos
Main Authors: Mukherjee, Somnath, Dutta, Debajyoti, Saha, Baisakhee, Das, Amit Kumar
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2008
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2564893/
https://ncbi.nlm.nih.gov/pubmed/18931438
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108027504
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