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Expression, purification, crystallization and preliminary X-ray diffraction studies of triosephosphate isomerase from methicillin-resistant Staphylococcus aureus (MRSA252)

Triosephosphate isomerase from methicillin-resistant Staphylococcus aureus (MRSA252) was cloned in pQE30 vector, overexpressed in Escherichia coli M15 (pREP4) cells and purified to homogeneity. The protein was crystallized from 1.6 M trisodium citrate dihydrate pH 6.5 using the hanging-drop vapour-d...

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Bibliografski detalji
Glavni autori: Mukherjee, Somnath, Dutta, Debajyoti, Saha, Baisakhee, Das, Amit Kumar
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2009
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2664771/
https://ncbi.nlm.nih.gov/pubmed/19342791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109010112
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