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Overproduction, purification and preliminary X-ray diffraction analysis of a sulfotransferase from Mycobacterium tuberculosis H37Rv

Sulfotransferase STF1 from the Mycobacterium tuberculosis H37Rv genome was overproduced in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffract to 1.5 Å resolution using synchrotron radiation at SPring-8. The crystals are monoclinic and be...

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Detalhes bibliográficos
Main Authors: Tanaka, Shotaro, Moriizumi, Yuuji, Kimura, Makoto, Kakuta, Yoshimitsu
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952402/
https://ncbi.nlm.nih.gov/pubmed/16508083
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309104022328
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