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Use of 2-D DIGE analysis reveals altered phosphorylation in a tropomyosin mutant (Glu54Lys) linked to dilated cardiomyopathy
Current electrophoretic methods have not been optimized to fully separate post-translationally modified mutant forms of tropomyosin (Tm) from wild-type cardiac samples. We describe here a method employing a modified 2-D PAGE/2-D DIGE protocol, to fully separate native, mutant (E54K), and phosphoryla...
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Main Authors: | , , , , , |
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Formáid: | Artigo |
Teanga: | Inglês |
Foilsithe: |
2008
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Ábhair: | |
Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2586826/ https://ncbi.nlm.nih.gov/pubmed/18095372 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.200700772 |
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