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Use of 2-D DIGE analysis reveals altered phosphorylation in a tropomyosin mutant (Glu54Lys) linked to dilated cardiomyopathy

Current electrophoretic methods have not been optimized to fully separate post-translationally modified mutant forms of tropomyosin (Tm) from wild-type cardiac samples. We describe here a method employing a modified 2-D PAGE/2-D DIGE protocol, to fully separate native, mutant (E54K), and phosphoryla...

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Main Authors: Warren, Chad M., Arteaga, Grace M., Rajan, Sudarsan, Ahmed, Rafeeq P. H., Wieczorek, David F., Solaro, R. John
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2008
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2586826/
https://ncbi.nlm.nih.gov/pubmed/18095372
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.200700772
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