Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8(+) T cells with peptide-major histocompatibility complex class I tetramers

The ability to quantify and characterize antigen-specific CD8(+) T cells irrespective of functional readouts using fluorochrome-conjugated tetrameric peptide-MHC class I (pMHCI) complexes in conjunction with flow cytometry has transformed our understanding of cellular immune responses over the past...

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Main Authors: Chattopadhyay, Pratip K., Melenhorst, J. Joseph, Ladell, Kristin, Gostick, Emma, Scheinberg, Philip, Barrett, A. John, Wooldridge, Linda, Roederer, Mario, Sewell, Andrew K., Price, David A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2586006/
https://ncbi.nlm.nih.gov/pubmed/18836993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cyto.a.20642
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