Multiplexed FRET to Image Multiple Signaling Events in Live Cells

We report what to our knowledge is a novel approach for simultaneous imaging of two different Förster resonance energy transfer (FRET) sensors in the same cell with minimal spectral cross talk. Previous methods based on spectral ratiometric imaging of the two FRET sensors have been limited by the av...

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Autori principali: Grant, David M., Zhang, Wei, McGhee, Ewan J., Bunney, Tom D., Talbot, Clifford B., Kumar, Sunil, Munro, Ian, Dunsby, Christopher, Neil, Mark A. A., Katan, Matilda, French, Paul M. W.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Biophysical Society 2008
Soggetti:
Biophysical Letters
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2576366/
https://ncbi.nlm.nih.gov/pubmed/18757561
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.139204
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