Fluorescence Lifetime Imaging Provides Enhanced Contrast when Imaging the Phase-Sensitive Dye di-4-ANEPPDHQ in Model Membranes and Live Cells

We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700 ps lifetime shift between liquid-disordered and liquid-ordered phases offers greater contrast than the 60 nm spectral shift previously reported. Detection o...

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Main Authors: Owen, Dylan M., Lanigan, Peter M. P., Dunsby, Christopher, Munro, Ian, Grant, David, Neil, Mark A. A., French, Paul M. W., Magee, Anthony I.
Format: Artigo
Jezik:Inglês
Izdano: Biophysical Society 2006
Teme:
Biophysical Letters
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1459501/
https://ncbi.nlm.nih.gov/pubmed/16617080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.084673
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