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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
We present an open source high content analysis instrument utilizing automated fluorescence lifetime imaging (FLIM) for assaying protein interactions using Förster resonance energy transfer (FRET) based readouts of fixed or live cells in multiwell plates. This provides a means to screen for cell sig...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5352269/ https://ncbi.nlm.nih.gov/pubmed/28190060 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55119 |
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