Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells

We describe an open-source automated multiwell plate fluorescence lifetime imaging (FLIM) methodology to read out Förster resonance energy transfer (FRET) between fluorescent proteins (FPs) labeling endogenous kinetochore proteins (KPs) in live budding yeast cells. The low copy number of many KPs an...

Full beskrivning

Sparad:
Bibliografiska uppgifter
I publikationen:SLAS Technol
Huvudupphovsmän: Guo, Wenjun, Kumar, Sunil, Görlitz, Frederik, Garcia, Edwin, Alexandrov, Yuriy, Munro, Ian, Kelly, Douglas J., Warren, Sean, Thorpe, Peter, Dunsby, Christopher, French, Paul
Materialtyp: Artigo
Språk:Inglês
Publicerad: SAGE Publications 2019
Ämnen:
Original Research
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC6537140/
https://ncbi.nlm.nih.gov/pubmed/30629461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/2472630318819240
Taggar: Lägg till en tagg
Inga taggar, Lägg till första taggen!

Liknande verk