Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells

We describe an open-source automated multiwell plate fluorescence lifetime imaging (FLIM) methodology to read out Förster resonance energy transfer (FRET) between fluorescent proteins (FPs) labeling endogenous kinetochore proteins (KPs) in live budding yeast cells. The low copy number of many KPs an...

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Detalhes bibliográficos
Publicado no:SLAS Technol
Main Authors: Guo, Wenjun, Kumar, Sunil, Görlitz, Frederik, Garcia, Edwin, Alexandrov, Yuriy, Munro, Ian, Kelly, Douglas J., Warren, Sean, Thorpe, Peter, Dunsby, Christopher, French, Paul
Formato: Artigo
Idioma:Inglês
Publicado em: SAGE Publications 2019
Assuntos:
Original Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6537140/
https://ncbi.nlm.nih.gov/pubmed/30629461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/2472630318819240
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