Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

We present an open source high content analysis instrument utilizing automated fluorescence lifetime imaging (FLIM) for assaying protein interactions using Förster resonance energy transfer (FRET) based readouts of fixed or live cells in multiwell plates. This provides a means to screen for cell sig...

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發表在:J Vis Exp
Main Authors: Görlitz, Frederik, Kelly, Douglas J., Warren, Sean C., Alibhai, Dominic, West, Lucien, Kumar, Sunil, Alexandrov, Yuriy, Munro, Ian, Garcia, Edwin, McGinty, James, Talbot, Clifford, Serwa, Remigiusz A., Thinon, Emmanuelle, da Paola, Vincenzo, Murray, Edward J., Stuhmeier, Frank, Neil, Mark A. A., Tate, Edward W., Dunsby, Christopher, French, Paul M. W.
格式: Artigo
語言:Inglês
出版: MyJove Corporation 2017
主題:
Biophysics
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5352269/
https://ncbi.nlm.nih.gov/pubmed/28190060
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55119
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