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Multiplexed FRET to Image Multiple Signaling Events in Live Cells

We report what to our knowledge is a novel approach for simultaneous imaging of two different Förster resonance energy transfer (FRET) sensors in the same cell with minimal spectral cross talk. Previous methods based on spectral ratiometric imaging of the two FRET sensors have been limited by the av...

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Detaylı Bibliyografya
Asıl Yazarlar: Grant, David M., Zhang, Wei, McGhee, Ewan J., Bunney, Tom D., Talbot, Clifford B., Kumar, Sunil, Munro, Ian, Dunsby, Christopher, Neil, Mark A. A., Katan, Matilda, French, Paul M. W.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: The Biophysical Society 2008
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2576366/
https://ncbi.nlm.nih.gov/pubmed/18757561
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.139204
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