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Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity
We outline a powerful method for the directed evolution of integral membrane proteins in the inner membrane of Escherichia coli. For a mammalian G protein-coupled receptor, we arrived at a sequence with an order-of-magnitude increase in functional expression that still retains the biochemical proper...
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| Main Authors: | , , , , , , |
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| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
National Academy of Sciences
2008
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2567449/ https://ncbi.nlm.nih.gov/pubmed/18812512 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0803103105 |
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