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Conceptual and methodological advances in cell-free directed evolution

Although cell-free directed evolution methods have been used to engineer proteins for nearly two decades, selections on more complex phenotypes have largely remained in the domain of cell-based engineering approaches. Here, we review recent conceptual advances that now enable in vitro display of mul...

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Detalhes bibliográficos
Publicado no:Curr Opin Struct Biol
Main Authors: Dodevski, Igor, Markou, George C., Sarkar, Casim A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4641812/
https://ncbi.nlm.nih.gov/pubmed/26093059
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.sbi.2015.04.008
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