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Use of experimental crystallographic phases to examine the hydration of polar and nonpolar cavities in T4 lysozyme

There is conflicting evidence as to whether cavities in proteins that are nonpolar and large enough to accommodate solvent are empty or are occupied by disordered water molecules. Here, we use multiple-wavelength x-ray data collected from crystals of the selenomethionine-substituted L99A/M102L mutan...

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Main Authors: Liu, Lijun, Quillin, Michael L., Matthews, Brian W.
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 2008
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2567155/
https://ncbi.nlm.nih.gov/pubmed/18780783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0806307105
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