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Use of Urea and Glycine Betaine to Quantify Coupled Folding and Probe the Burial of DNA Phosphates in Lac Repressor – Lac Operator Binding

Thermodynamic analysis of urea-biopolymer interactions and urea effects on folding of proteins and α-helical peptides shows that urea interacts primarily with polar amide surface. Urea is therefore predicted to be a quantitative probe of coupled folding, remodeling and other large-scale changes in t...

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Autors principals: Hong, Jiang, Capp, Mike W., Saecker, Ruth M., Record, M. Thomas
Format: Artigo
Idioma:Inglês
Publicat: 2005
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2546872/
https://ncbi.nlm.nih.gov/pubmed/16363803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi0515218
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