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Genome Mining in Streptomyces coelicolor. Molecular Cloning and Characterization of a New Sesquiterpene Synthase

[Image: see text] The terpene synthase encoded by the SCO5222 (SC7E4.19) gene ofStreptomyces coelicolor was cloned by PCR and expressed inEscherichia coli as an N-terminal-His(6)-tag protein. Incubation of the recombinant protein, SCO5222p, with farnesyl diphosphate (1, FPP) in the presence of Mg(II...

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Hlavní autoři: Lin, Xin, Hopson, Russell, Cane, David E.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2006
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2533732/
https://ncbi.nlm.nih.gov/pubmed/16669656
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja061292s
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