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Genome Mining in Streptomyces coelicolor. Molecular Cloning and Characterization of a New Sesquiterpene Synthase
[Image: see text] The terpene synthase encoded by the SCO5222 (SC7E4.19) gene ofStreptomyces coelicolor was cloned by PCR and expressed inEscherichia coli as an N-terminal-His(6)-tag protein. Incubation of the recombinant protein, SCO5222p, with farnesyl diphosphate (1, FPP) in the presence of Mg(II...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2006
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2533732/ https://ncbi.nlm.nih.gov/pubmed/16669656 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja061292s |
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