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Genome Mining in Streptomyces clavuligerus. Expression and Biochemical Characterization of Two Cryptic Sesquiterpene Cyclases, (−)-δ-Cadinene Synthase and (+)-T-Muurolol Synthase

Two presumptive terpene synthases of unknown biochemical function encoded by the sscg_02150 and sscg_03688 genes of Streptomyces clavuligerus ATCC 27074 were individually expressed in Escherichia coli as N-terminal-His(6)-tag proteins, using codon-optimized synthetic genes. Incubation of recombinant...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Hu, Yunfeng, Chou, Wayne K. W., Hopson, Russell, Cane, David E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3034312/
https://ncbi.nlm.nih.gov/pubmed/21276937
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2010.11.008
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