Genome mining in Streptomyces avermitilis: Cloning and characterization of SAV_76, the synthase for a new sesquiterpene, avermitilol

[Image: see text] The terpene synthase encoded by the sav76 gene of Streptomyces avermtilis was expressed in Escherichia coli as an N-terminal-His(6)-tag protein, using a codon-optimized synthetic gene. Incubation of the recombinant protein, SAV_76, with farnesyl diphosphate (1, FPP) in the presence...

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Hauptverfasser: Chou, Wayne K. W., Fanizza, Immacolata, Uchiyama, Takuma, Komatsu, Mamoru, Ikeda, Haruo, Cane, David E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2904616/
https://ncbi.nlm.nih.gov/pubmed/20536237
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja103087w
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