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Identification of 17- α-Ethynylestradiol Modified Active Site Peptides and Glutathione Conjugates Formed During Metabolism and Inactivation of P450s 2B1 and 2B6

The oral contraceptive 17-α-ethynylestradiol (17EE) is a mechanism-based inactivator of P450s 2B1 and 2B6. Inactivation of P450s 2B1 and 2B6 in the reconstituted system by [(3)H]17EE resulted in labeling of the P450 apo-protein. Mass spectral analysis of 17EE-inactivated P450 2B1 showed an increase...

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Bibliographic Details
Main Authors: Kent, Ute M., Lin, Hsia-lien, Mills, Danielle E., Regal, Kelly A., Hollenberg, Paul F.
Format: Artigo
Language:Inglês
Published: 2006
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2533710/
https://ncbi.nlm.nih.gov/pubmed/16485904
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/tx050256o
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