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Modification of Serine 360 by a Reactive Intermediate of 17-α-Ethynylestradiol Leads to Mechanism-based Inactivation of Cytochrome P450s 2B1 and 2B6

17-α-Ethynylestradiole (17EE) is a mechanism-based inactivator of CYP 2B1 and CYP 2B6 in the reconstituted system. The loss in enzymatic activity was due to the binding of a reactive intermediate of 17EE to the apoprotein. CYP 2B1 and CYP 2B6 were inactivated by 17EE and digested with trypsin. The p...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Kent, Ute M., Sridar, Chitra, Spahlinger, Greg, Hollenberg, Paul F.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2772146/
https://ncbi.nlm.nih.gov/pubmed/18729327
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/tx800138v
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