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Predicting failure rate of PCR in large genomes

We have developed statistical models for estimating the failure rate of polymerase chain reaction (PCR) primers using 236 primer sequence-related factors. The model involved 1314 primer pairs and is based on more than 80 000 PCR experiments. We found that the most important factor in determining PCR...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Andreson, Reidar, Möls, Tõnu, Remm, Maido
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2441781/
https://ncbi.nlm.nih.gov/pubmed/18492719
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn290
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