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Predicting failure rate of PCR in large genomes
We have developed statistical models for estimating the failure rate of polymerase chain reaction (PCR) primers using 236 primer sequence-related factors. The model involved 1314 primer pairs and is based on more than 80 000 PCR experiments. We found that the most important factor in determining PCR...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2008
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2441781/ https://ncbi.nlm.nih.gov/pubmed/18492719 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn290 |
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