Fluorescence correlation analysis of probe diffusion simplifies quantitative pathogen detection by PCR

A sensitive, labor-saving, and easily automatable nonradioactive procedure named APEX-FCS (amplified probe extension detected by fluorescence correlation spectroscopy) has been established to detect specific in vitro amplification of pathogen genomic sequences. As an example, Mycobacterium tuberculo...

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Main Authors: Walter, Nils G., Schwille, Petra, Eigen, Manfred
Format: Artigo
Jezik:Inglês
Izdano: The National Academy of Sciences of the USA 1996
Teme:
Biological Sciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC24001/
https://ncbi.nlm.nih.gov/pubmed/8917500
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