Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy

We have investigated the pH dependence of the dynamics of conformational fluctuations of green fluorescent protein mutants EGFP (F64L/S65T) and GFP-S65T in small ensembles of molecules in solution by using fluorescence correlation spectroscopy (FCS). FCS utilizes time-resolved measurements of fluctu...

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Détails bibliographiques
Auteurs principaux: Haupts, Ulrich, Maiti, Sudipta, Schwille, Petra, Webb, Watt W.
Format: Artigo
Langue:Inglês
Publié: The National Academy of Sciences 1998
Sujets:
Biological Sciences
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC24860/
https://ncbi.nlm.nih.gov/pubmed/9811841
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