Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy
We have investigated the pH dependence of the dynamics of conformational fluctuations of green fluorescent protein mutants EGFP (F64L/S65T) and GFP-S65T in small ensembles of molecules in solution by using fluorescence correlation spectroscopy (FCS). FCS utilizes time-resolved measurements of fluctu...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1998
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC24860/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9811841/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.23.13573 |
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