Fluorescence correlation spectroscopy reveals fast optical excitation-driven intramolecular dynamics of yellow fluorescent proteins

Fast excitation-driven fluctuations in the fluorescence emission of yellow-shifted green fluorescent protein mutants T203Y and T203F, with S65G/S72A, are discovered in the 10(−6)–10(−3)-s time range, by using fluorescence correlation spectroscopy at 10(−8) M. This intensity-dependent flickering is c...

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Detalles Bibliográficos

Publicado en:	Proc Natl Acad Sci U S A
Principais autores:	Schwille, Petra, Kummer, Susanne, Heikal, Ahmed A., Moerner, W. E., Webb, Watt W.
Formato:	Artigo
Idioma:	Inglês
Publicado:	National Academy of Sciences 2000
Assuntos:	Biological Sciences
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC26631/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10618386/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.1.151
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