Fluorescence correlation spectroscopy reveals fast optical excitation-driven intramolecular dynamics of yellow fluorescent proteins

Fast excitation-driven fluctuations in the fluorescence emission of yellow-shifted green fluorescent protein mutants T203Y and T203F, with S65G/S72A, are discovered in the 10(−6)–10(−3)-s time range, by using fluorescence correlation spectroscopy at 10(−8) M. This intensity-dependent flickering is c...

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Autores principales: Schwille, Petra, Kummer, Susanne, Heikal, Ahmed A., Moerner, W. E., Webb, Watt W.
Formato: Artigo
Lenguaje:Inglês
Publicado: The National Academy of Sciences 2000
Materias:
Biological Sciences
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC26631/
https://ncbi.nlm.nih.gov/pubmed/10618386
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