Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy

A method for sensitively monitoring enzyme kinetics and activities by using dual-color fluorescence cross-correlation spectroscopy is described. This universal method enables the development of highly sensitive and precise assays for real-time kinetic analyses of any catalyzed cleavage or addition r...

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Autores principales: Kettling, Ulrich, Koltermann, Andre, Schwille, Petra, Eigen, Manfred
Formato: Artigo
Lenguaje:Inglês
Publicado: The National Academy of Sciences 1998
Materias:
Biological Sciences
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC19026/
https://ncbi.nlm.nih.gov/pubmed/9465029
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