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Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy

We have investigated the pH dependence of the dynamics of conformational fluctuations of green fluorescent protein mutants EGFP (F64L/S65T) and GFP-S65T in small ensembles of molecules in solution by using fluorescence correlation spectroscopy (FCS). FCS utilizes time-resolved measurements of fluctu...

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Autori principali: Haupts, Ulrich, Maiti, Sudipta, Schwille, Petra, Webb, Watt W.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The National Academy of Sciences 1998
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC24860/
https://ncbi.nlm.nih.gov/pubmed/9811841
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