Novel computational methods for increasing PCR primer design effectiveness in directed sequencing

BACKGROUND: Polymerase chain reaction (PCR) is used in directed sequencing for the discovery of novel polymorphisms. As the first step in PCR directed sequencing, effective PCR primer design is crucial for obtaining high-quality sequence data for target regions. Since current computational primer de...

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Hlavní autoři: Li, Kelvin, Brownley, Anushka, Stockwell, Timothy B, Beeson, Karen, McIntosh, Tina C, Busam, Dana, Ferriera, Steve, Murphy, Sean, Levy, Samuel
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2008
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2396641/
https://ncbi.nlm.nih.gov/pubmed/18405373
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-9-191
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