Novel computational methods for increasing PCR primer design effectiveness in directed sequencing

BACKGROUND: Polymerase chain reaction (PCR) is used in directed sequencing for the discovery of novel polymorphisms. As the first step in PCR directed sequencing, effective PCR primer design is crucial for obtaining high-quality sequence data for target regions. Since current computational primer de...

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Hauptverfasser: Li, Kelvin, Brownley, Anushka, Stockwell, Timothy B, Beeson, Karen, McIntosh, Tina C, Busam, Dana, Ferriera, Steve, Murphy, Sean, Levy, Samuel
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2008
Schlagworte:
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2396641/
https://ncbi.nlm.nih.gov/pubmed/18405373
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-9-191
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