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Novel computational methods for increasing PCR primer design effectiveness in directed sequencing
BACKGROUND: Polymerase chain reaction (PCR) is used in directed sequencing for the discovery of novel polymorphisms. As the first step in PCR directed sequencing, effective PCR primer design is crucial for obtaining high-quality sequence data for target regions. Since current computational primer de...
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| Autores principales: | , , , , , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
BioMed Central
2008
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2396641/ https://ncbi.nlm.nih.gov/pubmed/18405373 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-9-191 |
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