Novel computational methods for increasing PCR primer design effectiveness in directed sequencing

BACKGROUND: Polymerase chain reaction (PCR) is used in directed sequencing for the discovery of novel polymorphisms. As the first step in PCR directed sequencing, effective PCR primer design is crucial for obtaining high-quality sequence data for target regions. Since current computational primer de...

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Autores principales: Li, Kelvin, Brownley, Anushka, Stockwell, Timothy B, Beeson, Karen, McIntosh, Tina C, Busam, Dana, Ferriera, Steve, Murphy, Sean, Levy, Samuel
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2008
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2396641/
https://ncbi.nlm.nih.gov/pubmed/18405373
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-9-191
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