Automated degenerate PCR primer design for high-throughput sequencing improves efficiency of viral sequencing

BACKGROUND: In a high-throughput environment, to PCR amplify and sequence a large set of viral isolates from populations that are potentially heterogeneous and continuously evolving, the use of degenerate PCR primers is an important strategy. Degenerate primers allow for the PCR amplification of a w...

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Bibliografiske detaljer
Main Authors: Li, Kelvin, Shrivastava, Susmita, Brownley, Anushka, Katzel, Dan, Bera, Jayati, Nguyen, Anh Thu, Thovarai, Vishal, Halpin, Rebecca, Stockwell, Timothy B
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2012
Fag:
Methodology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3548747/
https://ncbi.nlm.nih.gov/pubmed/23131097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-9-261
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