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Automated degenerate PCR primer design for high-throughput sequencing improves efficiency of viral sequencing

BACKGROUND: In a high-throughput environment, to PCR amplify and sequence a large set of viral isolates from populations that are potentially heterogeneous and continuously evolving, the use of degenerate PCR primers is an important strategy. Degenerate primers allow for the PCR amplification of a w...

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Detalhes bibliográficos
Main Authors: Li, Kelvin, Shrivastava, Susmita, Brownley, Anushka, Katzel, Dan, Bera, Jayati, Nguyen, Anh Thu, Thovarai, Vishal, Halpin, Rebecca, Stockwell, Timothy B
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3548747/
https://ncbi.nlm.nih.gov/pubmed/23131097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-9-261
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