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PrimerPooler: automated primer pooling to prepare library for targeted sequencing
Targeted next-generation sequencing based on PCR amplification involves pooling of hundreds to thousands of primers, for preamplification and subsequent parallel single/multiplex PCR. It is often necessary to allocate the set of primers into subpools, a common issue being potential cross-hybridizati...
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| Publicado en: | Biol Methods Protoc |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2017
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6994079/ https://ncbi.nlm.nih.gov/pubmed/32161789 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpx006 |
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