PrimerPooler: automated primer pooling to prepare library for targeted sequencing

Targeted next-generation sequencing based on PCR amplification involves pooling of hundreds to thousands of primers, for preamplification and subsequent parallel single/multiplex PCR. It is often necessary to allocate the set of primers into subpools, a common issue being potential cross-hybridizati...

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Bibliographic Details
Published in:Biol Methods Protoc
Main Authors: Brown, Silas S., Chen, Yun-Wen, Wang, Ming, Clipson, Alexandra, Ochoa, Eguzkine, Du, Ming-Qing
Format: Artigo
Language:Inglês
Published: Oxford University Press 2017
Subjects:
Methods Manuscript
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6994079/
https://ncbi.nlm.nih.gov/pubmed/32161789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpx006
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