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Automated degenerate PCR primer design for high-throughput sequencing improves efficiency of viral sequencing
BACKGROUND: In a high-throughput environment, to PCR amplify and sequence a large set of viral isolates from populations that are potentially heterogeneous and continuously evolving, the use of degenerate PCR primers is an important strategy. Degenerate primers allow for the PCR amplification of a w...
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| Главные авторы: | , , , , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
BioMed Central
2012
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3548747/ https://ncbi.nlm.nih.gov/pubmed/23131097 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-9-261 |
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