Novel computational methods for increasing PCR primer design effectiveness in directed sequencing

BACKGROUND: Polymerase chain reaction (PCR) is used in directed sequencing for the discovery of novel polymorphisms. As the first step in PCR directed sequencing, effective PCR primer design is crucial for obtaining high-quality sequence data for target regions. Since current computational primer de...

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Detalhes bibliográficos
Main Authors: Li, Kelvin, Brownley, Anushka, Stockwell, Timothy B, Beeson, Karen, McIntosh, Tina C, Busam, Dana, Ferriera, Steve, Murphy, Sean, Levy, Samuel
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2396641/
https://ncbi.nlm.nih.gov/pubmed/18405373
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-9-191
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