Novel computational methods for increasing PCR primer design effectiveness in directed sequencing

BACKGROUND: Polymerase chain reaction (PCR) is used in directed sequencing for the discovery of novel polymorphisms. As the first step in PCR directed sequencing, effective PCR primer design is crucial for obtaining high-quality sequence data for target regions. Since current computational primer de...

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מידע ביבליוגרפי
Main Authors: Li, Kelvin, Brownley, Anushka, Stockwell, Timothy B, Beeson, Karen, McIntosh, Tina C, Busam, Dana, Ferriera, Steve, Murphy, Sean, Levy, Samuel
פורמט: Artigo
שפה:Inglês
יצא לאור: BioMed Central 2008
נושאים:
Software
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC2396641/
https://ncbi.nlm.nih.gov/pubmed/18405373
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-9-191
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