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Crystallization and evaluation of hen egg-white lysozyme crystals for protein pH titration in the crystalline state

To observe the ionized status of the amino acid residues in proteins at different pH (protein pH titration in the crystalline state) by neutron diffraction, hen egg-white lysozyme was crystallized over a wide pH range (2.5–8.0). Crystallization phase diagrams at pH 2.5, 6.0 and 7.5 were determined....

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Bibliographische Detailangaben
Hauptverfasser: Iwai, Wakari, Yagi, Daichi, Ishikawa, Takuya, Ohnishi, Yuki, Tanaka, Ichiro, Niimura, Nobuo
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2008
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2394781/
https://ncbi.nlm.nih.gov/pubmed/18421167
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0909049507059559
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