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Crystallization and evaluation of hen egg-white lysozyme crystals for protein pH titration in the crystalline state

To observe the ionized status of the amino acid residues in proteins at different pH (protein pH titration in the crystalline state) by neutron diffraction, hen egg-white lysozyme was crystallized over a wide pH range (2.5–8.0). Crystallization phase diagrams at pH 2.5, 6.0 and 7.5 were determined....

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Detalhes bibliográficos
Main Authors: Iwai, Wakari, Yagi, Daichi, Ishikawa, Takuya, Ohnishi, Yuki, Tanaka, Ichiro, Niimura, Nobuo
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2394781/
https://ncbi.nlm.nih.gov/pubmed/18421167
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0909049507059559
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